The stains found on medieval manuscripts indicate a past life laden with human interactions over the course of centuries. Reading these signals using multispectral imaging—in concert with conventional information such as script, collation, illumination, and size—adds to our understanding of a manuscript’s history and use by bringing the invisible into the realm of the visible. The Library of Stains project, a one-year pilot study, has 1) published 220 Gb of imaging data in a stain database for scholars, librarians, and conservators 2) generated a methodological approach using multispectral imaging for the study of stains, and 3) provided a model for public-facing and interdisciplinary collaboration. The project’s data visualizations are hosted on the Digital Mappa platform, a University of Wisconsin homegrown and funded Digital Humanities resource for annotating and linking data. To learn more about the Library of Stains project, check out the University of Wisconsin’s February Research Data Services Newsletter, the Library of Stains Project in Zenodo, or the data visualizations in Digital Mappa.